I know that , for example , vaccinia viruses have been used in
transfer of foreign genes into cells. The gene is incorporated
into the viral genome by homologous recombination from a plasmid
if I am not wrong. Has anybody tried this or any other method to
incorporate genes into the herpes virus genome ? Which part of
the viral genome is targeted in general ? How stable would it
be (I mean the recombinant virus) ? What promoters for expression
of the foreign gene would be used ? How can we select for the
recombinant virus? ...
Thanks in advance.
