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Cervical Swabs for HPV PCR

Dan Mcleod aq224 at FreeNet.Carleton.CA
Wed Sep 21 15:21:46 EST 1994

I would like to hear from anyone who is actually doing PCR for Human
Papillomaviruses on DNA from cells obtained by cervical swabs, cytobrush,
cervical lavage etc.  Basically, what works best for you?  For example,
which do you recommend.. brush or swab or?,is the swab, brush etc. left dry
in collection tube?, in a transport media?, at what temperature?  How best
to separate cells?  extraction procedure? and so on.  
Many thanx for any good info.

Dan McLeod

Dan McLeod,
Nepean(Ottawa), Canada
mail dmcleod at hpb.hwc.ca
National Lab. Viral Oncology

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