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Polio cultivation

ijiwaru at wheel.dcn.davis.ca.us ijiwaru at wheel.dcn.davis.ca.us
Tue Feb 6 12:30:10 EST 1996

In article <9602061829.AA11995 at ariel.med.pitt.edu>, bap at MED.PITT.EDU
(Bruce Phillips) wrote:

>         HeLa cells are usually the easiest cell culture for the growth of
> polioviruses.  Be advised that types 1 and 2 strains give significantly
> higher yields than type 3 strains.  I believe this is true regardless of
> the cell type used.

Not all strains of HeLa cells will support poliovirus replication.  I
think it was the HeLa S3 from ATCC that would not support viral
replication in our hands.  Any primate cell line should be a suitable
host, CV-1, Cos-1, etc. as long as it has the receptor, for that matter
receptor-positive mouse cells should work.  As for the differential strain
yields, as I remember it, type 2 was the lowball in our hands, although it
could be because we had a mouse adapted strain of type 2.

Lyle Najita
Plant Pathology (I know, what does this have to do with polio?)
University of California - Davis

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