IUBio GIL .. BIOSCI/Bionet News .. Biosequences .. Software .. FTP

Mouse virus, NOT bone-marrow derived cells?

brett brett at borcim.wustl.edu
Tue Nov 12 13:48:39 EST 1996

>The lab that I did my grad work in does a lot of
>work with Sindbis virus.  If memory serves (and sometimes
>it doesn't...), SB didn't replicate in lymphoid cells.  Replication
>was pretty much confined to fibroblasts, skeletal muscle, and
>neural/glial type cells.  SB is relatively safe, grows to phenomenal
>titers in cell culture, and is easy to purify.  You might want
>to do a lit search to see if there is anything recent out there 
>that I have missed.  Names of authors to check would be:
>Johnston, R; Griffin, D; or Rice, C
>Peter C. Charles, PhD
>Department of Pathology (Neuropathology)
>Albert Einstein College of Medicine

I'd be careful. Sindbis can infect mononuclear cell lines of hematopoetic
origin, at least in vitro. It is unclear how important similar cell types
are  in vivo. Ian's question can't easily be answered in absolutes. How
much "leak" can he tolerate? I suppose one could use a (recombinant) virus
with a well-defined receptor on a target cell type, but you'd still go nuts
convincing anyone that no bone-marrow derived cells were infected.

Brett Lindenbach
Program in Immunology                              
Washington University - St Louis                  
brett at borcim.wustl.edu                             

"I own my own pet virus. I get to pet and name her." - Cobain

More information about the Virology mailing list

Send comments to us at archive@iubioarchive.bio.net