Department of Molecular Biology and
Genetics Johns Hopkins University
School of Medicine Ross Research
Bldg., Rm. 1050 720 N. Rutland Ave.
Baltimore, MD 21205
January 5th, 1997
To whom it may concern:
I am writing in regards to potential post-doctoral / entry level
positions that may be available in the areas of basic science AIDS
research and HIV vaccine development.
I am a 5th year Ph.D. candidate in the Biochemistry, Cellular and
Molecular Biology Training Program at the Johns Hopkins University
School of Medicine. I plan to complete my graduate program in the
spring/summer of 1997.
My research, under the direction of Robert F. Siliciano M.D. Ph.D.,
has been focused in two areas related to HIV/AIDS research. I
developed a novel method (AIDS Research and Human Retroviruses, 10:
1423, 1994) for the detection and evaluation of cytolytic T-cell
responses to candidate AIDS vaccines being tested in Phase I clinical
trials at the Center for Immunization Research here at Johns Hopkins
(J. Infect. Dis. 171: 1623, 1995). This method is now widely used in
the testing of AIDS vaccines.
More recently my thesis work has focused on developing a better
understanding of the role of a highly conserved YXXL internalization
motif within the envelope glycoprotein gp41 of HIV-1 in viral
assembly, host immune recognition, and CD4 cell loss (J. Virol.,
10:6547-6556 (1996)).
I seek a post doctoral / entry level position in the area of basic
sciences AIDS research and HIV vaccine development. My nine years
research experience, my excellent writing and verbal skills, along
with my proven ability to publish in peer reviewed journals makes me
an excellent candidate.
I welcome an opportunity to discuss my capabilities with respect to
opportunities that may exist in this area. I have enclosed a copy of
my curriculum vitae along with the name and addresses of three
references for your information.
Please feel free to contact me by mail at the above address or by
telephone or E-mail:
Phone: 410-955-7757
Fax: 410-955-0964
email: egan at welchlink.welch.jhu.eduhttp://www.welch.jhu.edu/~egan
Sincerely,
Michael A. Egan
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CURRICULUM VITAE
Name: Michael A. Egan
Current Appointment: Ph.D. Candidate, Graduate Program in
Biochemistry, Cellular and Molecular
Biology, Johns Hopkins University,
School of
Medicine,
Baltimore, MD
Office Address: Johns Hopkins University School of
Medicine,
Department of Medicine
Rm. 1050 Ross Research Bldg.
720 N. Rutland Ave.
Baltimore, MD 21205
Phone: 410-955-7757
Fax: 410-955-0964
e-mail: egan at welchlink.welch.jhu.eduhttp://www.welch.jhu.edu/~egan
Home Address: 3721 Rexmere Rd.
Baltimore, MD 21218
Phone 410-366-3268
EDUCATION
1992-present Ph.D. Candidate, Graduate Program in
Biochemistry, Cellular and Molecular
Biology (BCMB), Johns Hopkins
University, School of Medicine,
Baltimore, MD
1987-1992 B.S., Chemistry, Magna Cum Laude,
Northern Arizona University,
Flagstaff, AZ
HONORS AND AWARDS
Undergraduate Magna Cum Laude in Chemistry
Education Grade Point Average 3.80/4.00
NSF Summer Research Fellowship
Golden Key National Honor Society Phi
Lambda Upsilon Chemistry Honor Society
Phi Kappa Phi Honor Society Dean's
List seven semesters
Post Graduate Fellowship, Graduate Program in
Biochemistry, Education Cellular and Molecular
Biology (BCMB), Johns
Hopkins University School of Medicine
WORK EXPERIENCE
1988-1991 Undergraduate Chemistry lab teaching
assistant,
Northern Arizona University
Summer 1991 NSF Research Fellowship, University of
Georgia
1983-1987 Enlistment in the U.S. Marine Corps,
honorable
discharge.
RESEARCH EXPERIENCE
1992-Present Elucidating the role of the YXXL
internalization
motif within the envelope protein
(gp41) of HIV-1 in viral assembly,
immune
recognition, and
CD4 cell loss. Developed a novel
method,
now
widely used in the AIDS field, for the
detection
of (HIV-1)-specific CTL responses in
human vaccine recipients. In a Phase I
clinical
trial evaluated the ability of an
experimental HIV-1 vaccine to elicit
HIV-specific CTL responses in
sero-negative volunteers.
Laboratory of Robert F. Siliciano,
Department of Medicine, Johns Hopkins
University School of Medicine.
1991-1992 Using semi-empirical computational
methods to
calculate the structure and electronic
properties of the mono-boro-analogs of
acetylcholine.
Laboratory of Dr. Robert W. Zoellner,
Department of Chemistry, Northern
Arizona University
NSF 1991 Summer Mutagenesis of murine ferrochelatase
in looking Fellowship for molecular mimicry in
erythropoietic
protoporphyria.
Laboratory of Dr. Harry A. Dailey,
Department of Microbiology, University
of Georgia
1988-1992 The effect of endurance training on
glucose
metabolism.
Laboratory of Dr. Scott Savage and Dr.
Hans M.
Gunderson, Exercise Physiology Lab,
Northern Arizona University
SKILLS
Trained in standard molecular biology techniques, PCR, SDS-PAGE,
Western Blot, Pulse Chase Analysis, the use of Fluorescence Activated
Cell Sorter analysis (FACS), all aspects of human tissue culture, and
optimization and implementation of standard immunological assays (Cr51
release, CTL assays, ADCC, ELISA). Five years experience in the
handling and use of high titer infectious material (HIV-1, vaccinia
virus, EBV) under Biohazard Level 3 (P3) laboratory conditions.
Computer trained with experience programming in FORTRAN, C/C++, HTML.
Skilled in the use of DOS/Windows, Mac and Unix operating systems.
Able to efficiently access and publish information on the WWW and
Internet.
PUBLICATIONS
1. Michael A. Egan and Robert W. Zoellner. Structural and Electronic
Characteristics of the Monoboro-Analogs of the Acetylcholine Cation As
Determined by the Semiempirical MNDO Computational Method. J. Org.
Chem., 58:1719-1729 (1993).
2. M.N. Lubaki, Michael. A. Egan, Robert. F. Siliciano, Kent. J.
Wienhold, and Robert. C. Bollinger. A Novel Method for the Detection
and Expansion of HIV Type 1-Specific Cytolytic T Lymphocytes. AIDS
Res. Hum. Retroviruses, 10:1423-1427 (1994).
3. Michael A. Egan, Wendy A. Pavlat, James Tartaglia, Enzo Paoletti,
K. J. Wienhold, Mary Lou Clements, and Robert F. Siliciano. Induction
of Human Immunodeficiency Virus Type 1 (HIV-1)-Specific Cytolytic T
Lymphocyte Responses in Seronegative Adults by a Nonreplicating,
Host-Range-Restricted Canarypox Vector (ALVAC) Carrying the HIV-1MN
env Gene, J. Infect. Dis., 171:1623-1627 (1995).
4. Robert. C. Bollinger, Michael. A. Egan, Tae-Wook Chun, Bonnie
Mathieson, and Robert F. Siliciano. Cellular Immune Responses to HIV-1
in Progressive and Non-Progressive Infections. AIDS, 10:1-12 (1996).
5. Michael A. Egan, Lucy M. Carruth, Jennifer F. Rowell, Xiaofang Yu,
and Robert F. Siliciano. HIV-1 Envelope Protein Endocytosis Mediated
by a Highly Conserved Intrinsic Internalization Signal in the
Cytoplasmic Domain of gp41 Is Suppressed in the Presence of the
Pr55gag Precursor Protein, J. Virol., 70:6547-6556 (1996).
Abstracts
1. Michael A. Egan and Robert W. Zoellner. The Mono-Boro-Analogs of
Acetylcholine: Structural and Electronic Comparisons to the Parent
Acetylcholine Using the MNDO Computational Method. Annual American
Chemical Society Meeting, San Francisco, CA, April 1992.
2. Michael A. Egan, Kent Weinhold, Enzo Paoletti, James Tartaglia,
Mary Lou Clements, Wendy Pavlat, and Robert F. Siliciano. Cytotoxic T
Lymphocyte Responses to Recombinant Canarypox-gp160 MN (ALVAC vCP125,
HIV-1 gp160 MN) in HIV-1 Uninfected Adult Volunteers. The 6th Annual
Conference on Advances in AIDS Vaccine Development, Alexandria, VA,
October 30th - November 4th, 1993.
3. Michael A. Egan, Wendy Pavlat, James Tartaglia, Enzo Paoletti,
Robert F. Siliciano and Mary Lou Clements. Induction of
HIV-1-specific Cytotoxic T Lymphocyte Responses in Seronegative Adults
by a Non-Replicating, Host Range Restricted Recombinant Canarypox
Vector Carrying the HIV-1 env Gene. The 7th Annual Conference on
Advances in AIDS Vaccine Development, Reston VA, November 6th-10th,
1994.
4. Michael A. Egan, Wedy Pavlat, James Tartaglia, Enzo Paoletti, Mary
Lou Clements and Robert F. Siliciano. Cytotoxic T Lymphocyte
Responses to Recombinant Canarypox-gp160 MN (ALVAC vCP125, HIV-1 gp160
MN) in HIV-1 Uninfected Adult Volunteers. The Frank B. Polk Symposium
on AIDS, Baltimore, MD, April 15th-16th, 1994.
5. Michael A. Egan, Jennifer F. Rowell, Robert F. Siliciano and Lucy
M. Carruth. The Role of the gp41 (YXXL) Internalization Motif in HIV-1
Assembly.", Third Conference on Retroviruses and Opportunistic
Infections, Washington, DC, January 28th - February 2nd, 1996.
REFERENCES
Robert F. Siliciano, M.D., Ph.D.
Assistant Professor, Department of Immunology
Johns Hopkins University School of Medicine
Rm. 1049, Ross Research Bldg. 720 N. Rutland
Ave. Baltimore, MD 21205 Office: 410-955-2958
Fax: 410-955-0964 e-mail:
rsilicia at welchlink.welch.jhu.edu
Robert C. Bollinger, M.D., M.P.H.
Assistant Professor, Division of Infectious
Disease Johns Hopkins University School of
Medicine Rm. 1159, Ross Research Bldg. 720 N.
Rutland Ave. Baltimore, MD 21205 Office:
410-614-0936 Fax: 410-955-7889 e-mail:
rcb at welchlink.welch.jhu.edu
Xiao-Fang Yu, M.D., Ph.D.
Department of Molecular Biology and Immunology
Johns Hopkins University School of Hygiene and
Public Health 615 N. Wolfe, St. Baltimore, MD
21205 Phone: 410-955-3768 Fax:
410-955-0105
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Michael A. Egan
Department of Molecular Biology and Genetics
Ross Research Bldg., Rm. 1050
Johns Hopkins University
720 N. Rutland Ave.
Baltimore MD 21205
Phone: 410-955-7757
Fax: 410-955-0964
email: egan at welchlink.welch.jhu.eduhttp://www.welch.jhu.edu/~egan
