IUBio GIL .. BIOSCI/Bionet News .. Biosequences .. Software .. FTP

Increasing virus titer

Jeffrey Bunch jbunch at earthlink.net
Sun Oct 19 02:03:23 EST 1997

Enhancing virus attachment is of interest to me as I require the highest
infectious units I can obtain for my work.  I work with a variety of
animal and human viruses.  For example I can obtain 10 logs of EMC in
L929 cells but then I have difficulty even obtaining 7 logs of Porcine
Parvovirus in PK-13 cells. I am in great need of improving the culturing
conditions for PPV.  Besides adding polybrene and lowering the pH (for
enhancing virus attachment) do you have any suggestions?

More information about the Virology mailing list

Send comments to us at archive@iubioarchive.bio.net