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Vaccinia infected P815 cells in CTL assay

Koen Allosery koen_allosery at innogenetics.be
Mon Aug 30 15:41:52 EST 1999

We have some problems infecting suspension cells with Vaccinia virus.  
We are trying to infect the mouse mastocytoma cell line P815, to be used in
a CTL-assay.  
These cells can not be easily plated, and do not attach to flasks.

Has someone experience with infection of these cells with Vaccinia ?

Suggestions, advice and references to useful protocols are welcome !



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