RE: KUN and WNV
What about glycosylation of proteins from infected cell cultures -
absolutely nothing to do with DNA! Use tritiated mannose and separate on
SDS-PAGE. KUN has a set of glycoproteins - gp66, gp 59 & gp 53. Some
strains of WNV are not glycosylated (?? from memory) and the MW will be
different for those that are!
>From a flavivirus protein dinosaur!!
Dr Geoff Crawford
89 Dellfield Drive
Templestowe VIC 3106
Phone/Fax: +61 3 981 272 80
Mobile +61 412 599 649
Email access_academix at optusnet.com.au
jason roberts <pteropid1 at ozemail.com.au> wrote in message
news:GZKn6.960$o4.48244 at ozemail.com.au...
> Hi everyone,
>> Just another inquiry into diagnostic testing,
>> Given the remarkably close relationship between the Kunjin virus and
> Westnile virus genetic structure, I was wondering if anyone knows of a
> simple method for differentiating between these two viruses (without
> resorting to sequencing).
>> We currently use a PCR detection method, based on universal primers
> at the NS5 gene (Chang et. al. C.D.C ). I feel that it would be of benefit
> to be able to distinguish between the two viruses...
>> ....perhaps someone has come up with a probe for each type??
>> >From direct observation of the genetic data, it seems like an accurate
> differentiation is going to be rather elusive, without resorting to more
> complex methodology.
>> Many thanks.
>> Jason A. Roberts
> Virology Division,
> Department of Microbiology.
> Prince of Wales Hospital.
>Robertsjas at sesahs.nsw.gov.au>>>