Hi,
I use seaplaque agarose from FMC for overlay for BHV-1 plaque assay.
You can microwave the agarose(which is sufficient) and mix with 2x DMEM or
similar medium with anitbiotic. I have adapted the protocol from :
http://www.invitrogen.com/Content/Tech-Online/molecular_biology/manuals_pps/maxbacte_man.pdf
(Page22-)
You can also try this web site:
http://www.virapur.com/new2431.html
And there is a software also available for the plaque assay @
http://www.androclus.vet.uu.nl/spotter/spotter.html
Bye,
Gopinath
===========
Gopinath Raju Seetharaman
Post-Doctoral Research Fellow,
University of Nebraska-Lincoln,
Dept. of Veterinary and Biomedical Sciences,
East Campus Loop and Fair street,
Lincoln,NE 68583.
Tel:402-472-3320
>From: "Frank M. Mattes" <mattes at rfhsm.ac.uk>
>To: bionet-virology at net.bio.net>Subject: plaque assay protocoll ?
>Date: Thu, 3 May 2001 14:19:35 +0100 (BST)
>>Dear newsgroup reader,
>>does anybody know a good protocoll to do a plaque assay for cmv ?.
>I know you need methylcellulose, wich is critical to avoid the spread of
>the
>virus within the media.
>I'm wondering also if these clellulose media is also pre-mixed available
>from a commerical source.
>>Many thanks for your help
>>Frank Mattes
>Dept. of Virology
>RFH
>London
>>
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