We've done slowcooling with as little as 50% of the model. It's
important to make sure the ends don't move too far. I would recommend
using harmonic point restraints to restrain the ends of the polypeptide
chain (terminii and missing pieces of the model).
Axel Brunger
> Does anyone have experience using simulated annealing during the
> course of model-building: specifically, what fraction of the
> coordinates ought to be in place before slow-cooling is attempted?
> Any practical advice would be appreciated.
>
