Rob Beynon's nice BufferStack is now available from the IuBio archives via anonymous FTP: >ftp iubio.bio.indiana.edu ftp> user anonymous guest ftp> cd [archive.molbio.mac] ftp> get buffer.sit_hqx Given the appropriate information, the BufferStack will construct a complete recipe for a buffer that is defined in terms of both pH and ionic strength, at the temperature of use. The recipe will be based on the simplest method of preparing a buffer- by titration of a solution of acid or base to the correct pH. Author: Rob Beynon Proteolysis Research Group Department of Biochemistry University of Liverpool PO Box 147, Liverpool L69 3BX, UK (SB06@UK.AC.LIV.IBM) (or try SB06@IBM.LIV.AC.UK from USA nets) See Beynon, R.J. (1988) "A Macintosh Hypercard stack for calculation of thermodynamically-corrected buffer recipes". CABIOS 4, 487-490