DNAdraw 2.50 October 24, 1995 The new version: Version 2.50 has the following changes from the previous version (2.26): 1) A great deal of work has been done to eliminate crashes; many errors corrected. (Thanks to Jim Goding.) 2) Automatic highlighting of alignment data now allows for equivalence matching of protein data, i.e. amino acids do not need to match exactly to be highlighted. (Thanks to many who asked for it.) 3) The Special menu has a new option for translation, which includes reverse-complement translation. Before, translation could only be done with the Format option. (Thanks to Susan Douglas.) 4) The manual has been revised, with new examples added. Six pages added. Feedback is always welcome. That's how I make DNAdraw better. What DNAdraw is and does: DNAdraw is a Macintosh program designed for preparing DNA and protein sequence data for publication. A large selection of types of highlighting options is available, including: dotted characters solid and dashed outlines 8 levels of shading different fonts and sizes bold, italic, and underline styles reverse font Unless otherwise selected, all text uses a fixed-space font (Courier) so that sequences line up. A proportional font can be used for annotation (such as restriction sites), and greek characters and sub and superscripts are available. The files used and created by DNAdraw are all text files (i.e. all ASCII characters). Output for DNAdraw (from the Print command in the File menu), is written to a PostScript file, which the user downloads to a PostScript printer. DNAdraw runs with the typical Macintosh interface, i.e. pull down menus, mouse control, scroll bars, dialog boxes, cut and paste, etc. The user selects text with the mouse, then chooses a type of highlighting or a special option from a menu. Rectangular areas of text are selected for highlighting (as opposed to line-oriented selection), and more than one region can be selected before a highlight type is specified. Normal Macintosh-type editing can be done by switching to Mac Edit mode. In addition to doing highlighting, DNAdraw has special features for formatting raw data into a style commonly used for publication, and for doing automatic highlighting of aligned sequences based on matching or equivalent characters in columns. The 46 page DNAdraw manual, in MicroSoft Word 5.1a format, contains many examples. The manual contains PostScript code for some of the figures, which means that those figures will appear (1) as PostScript code when displaying them, (2) as blank space when in Print Preview mode, and (3) correctly, when printed. Marvin Shapiro mbs@kias.com