The author of this program is Dietmar Tietz (Dietmar.Tietz@uni-giessen.de). Please contact him for questions or suggestions. ElphoFit 2.31 is freeware and was developed at the National Institutes of Health, Bethesda, Maryland, USA. ElphoFit is scientific computer software for the evaluation of electrophoretic mobility data based on linear or nonlinear Ferguson plots [log(mobility) vs. polymer concentration]. The program uses a Newton-Gauss Marquardt-Levenberg curve-fitting algorithm and a mathematical approach based on the "random space walk of a particle through a fiber network" (extended Ogston model). The program allows one to estimate particle- as well a polymer-specific parameters. Output is in the format of tables (including statistical parameters) and graphics. The program package provides samples of data, log and pict files as well as a Read-me Introduction with references to published articles which explain the underlying rationales and the operation of the program in great detail. All references are available upon request from the program author. ElphoFit 2.31 has been used in application to proteins, small and large DNA, viruses, conjugated vaccines and polystyrene particles (used as size standards). Mobility data originating from ordinary, capillary and Serwer-type 2-D agarose electrophoresis have been evaluated.