Gel Frag Sizer  Version 1.4
March 6, 1989  Don Gilbert
("README.DOC" added 3/6/89 by Spencer Yeh for BIONET distribution)


INTRODUCTION

Gel Frag Sizer is a Macintosh HyperCard stack which calculates restriction 
fragment sizes from their mobilities.  Two methods for estimating sizes are 
provided: the local reciprocal method of Elder & Southern or the cubic spline 
method.  The program provides a graphic plot of the standards and unknowns, as 
well as a tabular output.  Data from the program, including graphs, can be 
printed out or the numerical data can be saved to an ASCII text file.  The 
program is both user-friendly and quite well documented with help text and 
literature references.  


CONTACT ADDRESS

  Don Gilbert
  Biology Dept.
  Indiana Univ.
  Bloomington, IN  47405

  e-mail:  gilbertd@gold.bacs.indiana.edu  (Internet)
           gilbertd@IUBACS.bitnet   (Bitnet)


SYSTEMS SUPPORTED

Mac Plus, SE, and Macintosh II.  HyperCard is required.


AVAILABILITY

This program is available by anonymous FTP from BIONET (net.bio.net) in
the directory ~ftp/public/mac/gelfragsizer or, for BIONET subscribers only, by
postal mail from the BIONET Lending Library.  If you are a BIONET
subscriber and would like to receive the GELFRAGSIZER diskette by mail,
please send a stamped, self-addressed return envelope along with a
formatted diskette (specify capacity) and your request to:


	BIONET Administrator
	BIONET/ IntelliGenetics, Inc.
	700 East El Camino Real, Suite 300
	Mountain View, CA  94040

	tel.:  (415) 962-7337



SOURCE CODE

Source code is not available.


PROGRAM FILES before un-STUFFing (177 Kb):

readme.doc              This documentation file.
gelfragsizer.hqx        Stuffed and Binhexed archive file of the
                        Gel Frag Sizer files.   (168 Kb).


PROGRAM FILES after un-STUFFing:

Gel Frag Sizer          HyperCard stack  (173 Kb).
gelfragsizer.doc        Original notes on Gel Frag Sizer.


DOCUMENTATION

The Gel Frag Sizer HyperCard stack is internally documented through the use of 
the HyperCard "?" button.  Card-specific documentation provides a description 
of all of the HyperCard buttons as well as references for the two algorithms 
used to calculate sizes.


DEARCHIVING THE PROGRAM

".hqx" files must be first un-BinHexed.  To do this, use the "Other"
menu within the StuffIt program to choose the "Decode BinHex File..."
option or use the BinHex program's "File" menu to choose the "Download
-> Application" option.

A StuffIt ".sit" archive file can usually be opened simply by
double-clicking on the document's icon.  If this doesn't work, open up
the archive file from within the StuffIt application by using the "Open
Archive..." command in the "File" menu.


STARTING THE PROGRAM

A HyperCard stack can either be opened by double-clicking the Gel Frag Sizer 
icon, or by opening the stack from within HyperCard by using the "Open 
Stack..." option from the "File" menu.