CFRDBPH9.TCL TCL script for Beckman Coulter Biomek 2000 http://www.beckmancoulter.com/products/instrument/automatedsolutions/biomek/biomek2000_inst_dcr.asp 10/26/01 For full details, see: Lappin S; Cahlik J; Gold B (2001) Robot Printing of Reverse Dot Blot Arrays for Human Mutation Detection The Journal of Molecular Diagnostics 3, 178-188. ************************************************************************ This Tool Command Language (Tcl) script will control the Biomek 2000 for spotting oligonucleotide probes on to filter membranes to make reverse dot blot probe arrays. Under BioWorks 2.0, Tcl scripts are run through a BioScript file, which is accessed by the BioWorks software. The Tcl file is placed in the default directory, usually the BioWorks directory. A BioScript file is written as follows: GF CFRDBPH9.TCL (This command loads the file) TC SOURCE CFRDBPH9.TCL (This command executes the file) (See the BioScript reference in the BioWorks Software Reference Manual for an in depth explanation.) Save this BioScript file. The BioScript file which contains the Tcl script is executed through a BioWorks method. The BioWorks method should contain three steps. The first step sets up the initial configuration and generates the reminder screen upon starting the method. This will show the location of tools, tips and labware. Upon accepting the configuration, the second step is executed, consisting of an Insert BioScript function command that designates the BioScript file created above (see BioWorks Software Reference Manual). The execution of the BioScript file will load and run the Tcl file. The third step is an END step and is executed after the BioScript is complete and is required in all BioWorks methods. ************************************************************************